Prep HPLC Column, Bidentate C8, 4um, 100A. 21.2mm ID x 100mm Length. Cogent TYPE-C Brand. 1 EA.
Additional Info:
The 40008‑P21‑100 Cogent Bidentate C8™ Preparative HPLC Column is a mid‑length, large‑diameter preparative column designed for purification workflows requiring both high sample capacity and improved resolving power. Built on Cogent TYPE‑C™ silica hydride technology, this column delivers excellent selectivity, fast equilibration, and very robust column lifetime under demanding preparative conditions.
The stationary phase consists of an aliphatic C8 hydrocarbon chain bonded via two direct silicon–carbon bonds (bidentate attachment) to a silica hydride surface. This chemistry produces a phase that is more hydrophobic than conventional C8 columns, yet less hydrophobic than Bidentate C18™, making it ideal for hydrophobic molecules, metabolites, and amine‑containing analytes when C18 is overly retentive.
With a 21.2 mm internal diameter and 100 mm length, this column provides increased plate count and improved separation efficiency compared to short 50 mm preparative formats. This enables better resolution of closely eluting compounds while still supporting large injection volumes and high mass loads for preparative purification.
System Note: A specialized preparative HPLC system (adequate high‑flow pump capacity, injector, tubing/valving, and fraction‑collection hardware) is required for columns of this size.
Why Choose the 21.2 × 100 mm Prep Bidentate C8™ Format
This configuration is typically selected when:
C18 columns are too retentive for preparative targets
Greater resolution is required than 21.2 × 50 mm columns provide
Large sample loads require better separation before collection
Hydrophobic or basic analytes benefit from moderated retention
The 100 mm length increases interaction time and separation power, while TYPE‑C™ silica hydride chemistry maintains durability and fast equilibration.
Key Features
Cogent TYPE‑C™ Silica Hydride Technology
Non‑traditional reversed‑phase chemistry
Reduced silanol activity and improved peak shape
Faster equilibration than conventional preparative C8 columns
Very robust lifetime for repeated purification cycles
Bidentate C8™ Bonding
Two‑point attachment via direct Si–C bonds
Balanced hydrophobic retention
Ideal when C18 is overly retentive
Preparative Geometry with Enhanced Resolution
21.2 mm ID × 100 mm length
Higher plate count vs. shorter prep columns
Supports improved fraction purity and separation
Broad Method Compatibility
Stable at very low pH (below 2)
Compatible with aqueous‑rich mobile phases
Suitable for aggressive preparative workflows
Typical Applications
Preparative purification and fraction collection
Hydrophobic molecule isolation
Metabolite purification
Amine‑containing analytes
Scale‑up from analytical or shorter preparative methods
Situations where C18 retention is excessive
Made‑to‑Order & Pricing Information
This is a custom, made‑to‑order item.
Orders cannot be canceled once placed
Custom items are non‑returnable